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This air displacement step is critical: injecting liquid into a sealed vial without removing air first creates positive pressure that forces peptide solution back through the needle during withdrawal, wasting product and contaminating the needle exterior

This work was supported by the Lundbeck Foundation (fellowship R238-2016-2859), the BioInnovation Institute (grant number NNF20OC0063538) and the Novo Nordisk Foundation (grant numbers NNF17OC0026114 and NNF22OC0073778)

Effects of glucagon, catecholamines, and adenosine 3,5-monophosphate on gluconeogenesis in the perfused rat liver

These nutrients play important roles in maintaining normal male fertility